Synthesis and biological properties of triazole-linked locked nucleic acid.
نویسندگان
چکیده
We have synthesized and studied the biological and biophysical properties of triazole-linked ribo and xylo locked nucleic acid (LNA). The combination of LNA with the Isobe triazole linkage gave high binding affinity when incorporated at the 3' or 5' termini of oligonucleotides, but low binding affinity at internal positions. Antisense oligonucleotides (ASOs) and siRNAs containing triazole dimers were highly active and nuclease resistant. Surprisingly, the xyloLNA-modified siRNA was the most active of the series.
منابع مشابه
Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7cc05159j
Oligonucleotides containing internal triazole-3'-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified oligonucleotides, and significantly better than oligonucleotides containing triazole alone. In contrast LNA on the 5'-side of the triazole does not stabilise duplexes. Triazole-LNA confers great resistance towards enzymatic degradation relative to LNA alone.
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ورودعنوان ژورنال:
- Chemical communications
دوره 53 63 شماره
صفحات -
تاریخ انتشار 2017